Phenolyzer

Phenolyzer is a software that implements phenotype-based prioritization of candidate genes for human diseases.

Introduction

Prior biological knowledge and phenotype information may help pinpoint disease contributory genes in whole genome/exome sequencing studies on human diseases. We developed a computational tool called Phenolyzer, which follows a biologist's natural thought processes through four steps: term interpretation, seed gene generation, seed gene growth and data integration. Compared to competing approaches, Phenolyzer has superior performance on finding known disease genes, and on prioritizing recently published novel disease genes.

Releases

Phenolyzer is also available as a web-sever at here.

Mannual

The more detailed manual is available at here

Dependency

Bioperl

Graph::Directed

Installation

Please clone the repository into your computer:

git clone https://github.com/WGLab/phenolyzer

Then enter phenolyzer directory:

cd phenolyzer

Use your own Addon databases

Put your own addon databases into lib/compiled_database. For details of how to use your own Addon databases, please refer to FAQ

Use

-addon [Gene-disease database file name, delimited by comma] -addon_weight [default:1] -addon_gg [Gene-gene database file name, delimited by comma] -addon_gg_weight [default:1]

Pre-requisites

• Download the databases for CNV annotation (No need to do this for other functions)

perl bin/annotate.pl -downdb -buildver hg19 -webfrom annovar refGene lib/humandb
perl bin/annotate.pl -downdb -buildver hg18 -webfrom annovar refGene lib/humandb

Synopsis

• Print help message

perl disease_annotation.pl --help

• Prioritize 'Alzheimer' genes:

perl disease_annotation.pl alzheimer -prediction -phenotype -logistic -out ex1

• Prioritize 'Amyotrophic lateral sclerosis' genes:

disease_annotation.pl "Amyotrophic lateral sclerosis" -prediction -phenotype -logistic -out ex2

• Use the disease terms in 'example_disease.txt' file (without phenotype expansion):

perl disease_annotation.pl example_disease.txt -file -prediction -logistic -out ex3

• Use the phenotype terms in 'example_phenotype.txt' file:

perl disease_annotation.pl example_phenotype.txt -file -prediction -phenotype -logistic -out ex4

• Use the HPO terms in 'example_hpo.txt' file:

perl disease_annotation.pl example_hpo.txt -file -prediction -phenotype -logistic -out ex5 -addon DB_DISGENET_GENE_DISEASE_SCORE,DB_GAD_GENE_DISEASE_SCORE -addon_weight 0.25

• Use the cnv.bed region:

perl disease_annotation.pl alzheimer -bedfile cnv.bed -p -ph -logistic -out ex6

• Use the Mentha gene-gene interaction database as Addon

perl disease_annotation.pl alzheimer -p -ph -logistic -out ex7 -addon_gg DB_MENTHA_GENE_GENE_INTERACTION -addon_gg_weight 0.05

• To generate exactly the same result as Phenolyzer web server default settings

perl disease_annotation.pl alzheimer -p -ph -logistic -out ex8 -addon DB_DISGENET_GENE_DISEASE_SCORE,DB_GAD_GENE_DISEASE_SCORE -addon_weight 0.25

• Integrate with wANNOVAR output to prioritize variant

perl calculate_score.pl <phenolyzer_gene_list> <wannovar_genome_summary.txt>

• Input multiple diseases (alzheimer and brain)

perl disease_annotation.pl "alzheimer;brain" -p -ph -logistic -out ex9

• Parallelize phenotype processing to speed up calculations (fork subprocesses)

perl disease_annotation.pl "alzheimer;brain" -p -ph -logistic -nproc 2

• Use precalculated HPO term expansion and gene scores to speed up calculation. This requires the unzip command to be available in your system. Alzheimer and brain would be processed in parallel and the HPO terms would be pulled from the DB included in phenolyzer.

perl disease_annotation.pl "alzheimer;brain;HP:0004322;HP:0002240" -p -ph -logistic -nproc 2 -use_precalc

License Agreement

By using the software, you acknowledge that you agree to the terms below:

For academic and non-profit use, you are free to fork, download, modify, distribute and use the software without restriction.

For commercial use, you are required to contact Stevens Institute of Innovation at USC directly to discuss licensing options.

THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE.

Contact

• Hui Yang ([email protected])
• Kai Wang ([email protected])