Preprocessing on Brain MRI Sequence
This is a pipeline to do preprocessing on brain MR images of ADNI dataset
by using FMRIB Software Library (FSL) and Advanced Normalization Tools (ANTs).
1. Install FSL & ANTs
Download and install FSL as instructions here.
Compile ANTs from source code in Linux and macOS, or in Windows 10.
2. Install Python Packages
All required libraries are listed as below:
- tqdm
- numpy
- scipy
- nipype
- nibabel
- matplotlib
- sciKit-fuzzy (optional)
- scikit-learn (optional)
3. Download Dataset
The dataset used in this repo is AD and NC screening images of ADNI1 and ADNI2.
See README.md in data.
Here is one sample of original image.
4. Reorgnization Files
Switch the working directory to src. Run reorgnize.py, which merge ADNI1 and ADNI2 into one folder.
python reorgnize.py
5. Registration
Run registraion.py to transform images into the coordinate system of template by FSL FLIRT.
python registraion.py
The output of the above image from this step looks like:
6. Skull-Strpping
Run skull_stripping.py to remove skull from registrated images by FSL BET.
python skull_stripping.py
Output:
7. Bias Field Correction
Run bias_correction.py to remove bias-field signal from images by ANTs.
python bias_correction.py
Output:
8. Enhancement (optional)
Based on outputs from step 7, run enhancement.py to enhance images by histogram equalization.
python enahncement.py
9. Tissue Segmentation (optional)
Based on outputs from step 7, run segment.py to segment brain into GM, WM and CSF
by KMeans or Fuzzy-CMeans (you should change settings in script).
python segment.py
Or run fast_segment.py to do segmentation by FSL FAST.
python fast_segment.py