simple_sim_fusion_demo

Simple demo of structured illumination microscopy image fusion via Richardson-Lucy deconvolution. To run the code yourself, download sim_fusion.py and np_tif.py, put them in the same directory, and execute them in a Python 3 environment that includes Numpy and Scipy. To see the results of running the code, scroll down.

Given a 2D x-z object:

Illuminated with a series of 2D x-z intensity patterns like this:

And blurred with a 2D x-z PSF like this:

Yielding simulated data like this:

We process this simulated data into an estimate of the true density (truth is in red, estimate is in green):

Via iterative Richardson-Lucy deconvolution:

This work is licensed under a Creative Commons Attribution 4.0 International License.