Top 6 16-bit open source projects

Assembly-Lib
A 16-bits x86 DOS Assembly library that provides many useful functions for developing programs. It has both VGA grapics functions as well as general purpose utilities. The main purpose of this library was to be able to implement simple DOS games (in Assembly) using VGA (320x200, 256 colors) display.
QNICE-FPGA
QNICE-FPGA is a 16-bit computer system for recreational programming built as a fully-fledged System-on-a-Chip in portable VHDL.
retrogardens
16bit retro programming in DosBox with Borland C++ 3.1, Turbo Pascal 7 and TASM
semblance
Disassembler for Windows executables. Supports 16-bit NE (New Executable), MZ (DOS), and PE (Portable Executable, i.e. Win32) files.
asm16 projects
My small projects writen in 16 bit asm (NOTE: those are my practice projects that I wrote when I was 15, I give no warranty for this code!)
1-6 of 6 16-bit projects