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All Projects → gear-genomics → indigo

gear-genomics / indigo

Licence: GPL-3.0 license
Indigo: SNV and InDel Discovery in Chromatogram traces obtained from Sanger sequencing of PCR products

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Labels

sequencingvariantsalignmentcrispr-analysisindigovariant-callingpcrindelscrispr-cas9sanger-chromatogramsindel-discoveryallelesgear-genomicssanger-sequencingpcr-productschromatogram-traces

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Indigo is a rapid single-nucleotide variant (SNV) and insertion/deletion (InDel) discovery method in Chromatogram traces obtained from Sanger sequencing of PCR products. It can separate a mutated and wildtype allele and aligns both alleles against a reference sequence or wildtype chromatogram. Indigo discovers mutations generated by genome editing tools such as CRISPR/Cas9 or TALENs.

Indigo Online Method

Indigo can be run online as a web application at https://www.gear-genomics.com/indigo.

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